Seven Tips to Make PCR Primers Last Longer and PCR Reactions Run Better - Genetic Engineering News
23 May 2012, 2:33 pm
The Tris and EDTA prevent acidic water and contaminating DNases from hydrolyzing and enzymatically degrading DNA, respectively. 3. Aliquot the resuspended primers into working stocks. This eliminates the need for damaging freeze / thaw cycles of the master ...

Twelve Positions in a β-Lactamase That Can Expand Its Substrate Spectrum with a Single Amino Acid Substitution - Elites TV
22 May 2012, 9:16 pm
By analyzing 516 mutated enzymes that acquired the ceftazidime-hydrolyzing activity, we found twelve positions with single amino acid substitutions (altogether twenty-nine different substitutions), co-localized at the active-site pocket area. The ...